Search results for "Iceberg lettuce"

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Application of propidium monoazide quantitative PCR for selective detection of live Escherichia coli O157:H7 in vegetables after inactivation by esse…

2012

The use of propidium monoazide (PMA) is enjoying increased popularity among researchers in different fields of microbiology. Its use in combination with real-time PCR (qPCR) represents one of the most successful approaches to detect viable cells. PMA-qPCR has successfully been used to evaluate the efficacy of various disinfection technologies in different microorganisms. Initially, in this study the effect of four essential oils (EOs), cumin, clove, oregano and cinnamon, was evaluated on suspensions of the enterohemorrhagic Escherichia coli O157:H7 by PMA-qPCR, LIVE/DEAD BacLight flow cytometry analysis (LIVE/DEAD-FCM), and plate count. E. coli O157:H7 cells treated with EOs at killing conc…

AzidesMicroorganismFood ContaminationBiologymedicine.disease_causeEscherichia coli O157Real-Time Polymerase Chain ReactionMicrobiologyPolymerase Chain ReactionFlow cytometryMicrobiologyPropidium monoazideOriganumVegetablesmedicineEscherichia coliOils VolatileEscherichia coliIceberg lettucemedicine.diagnostic_testGeneral MedicineContaminationLettuceFlow CytometryDisinfectionReal-time polymerase chain reactionFood productsFood SciencePropidiumInternational journal of food microbiology

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PEPhysical Sciences and Engineering
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LSLife Sciences
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SHSocial Sciences and Humanities
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